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    Nikon algorithms fiji imagej nih
    Algorithms Fiji Imagej Nih, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej%2C+nis-elements/NIS-Elements/pm41861814-221-189-197
    Average 99 stars, based on 39764 article reviews
    algorithms fiji imagej nih - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Hypoxia restores the acidosis-induced inhibition of cancer cell dissemination.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Myr-HA-Akt1 (Ramaswamy et al.)49 Addgene, plasmid # 9008 mCherry-CRY2-iSH2 (Idevall Hagren O et al.)39 Addgene, plasmid # 66839 Software and algorithms ImageJ NIH https://imagej.net/software/fiji/ NIS-Elements Nikon https://www.microscope.healthcare.nikon.com/ products/software/nis-elements/softwareresources MATLAB (R2025b) MathWorks https://www.mathworks.com/products/ matlab.html bioRender bioRender https://www.biorender.com/ Seahorse Wave Desktop Agilent https://www.agilent.com/en/product/cell- analysis/real-time-cell-metabolic-analysis/ xf-software/seahorse-wave-desktop-software740897 Prism 10 GraphPad https://www.graphpad.com HCI software Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ cameras/software/imaging-software/U11158.html QuPath Bankhead et al.50 https://qupath.github.io/ Cell Reports 45, 116970, February 24, 2026 19 Unless otherwise indicated, the cell culture medium with prescribed pH was prepared as previously described.9,15,25 In brief, cell culture medium was supplemented with 25 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES; 15630080, Gibco) and 25 mM PIPES; P1851, Millipore Sigma), and pH was adjusted to 7.4 or 6.4 with addition of NaOH and HCl. .. In select experiments (Figures 2F, 2G, S4G; Figures S5A–S5C), an isotonic medium made from DMEM without NaHCO3 (12800-058, Gibco) and supplemented with either 16.5 mM NaHCO3, 20 mM NaCl (pHe = 7.4) or 2 mM NaHCO3, 2 mM HCl and 30 mM NaCl (pHe = 6.4) was used.

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Article Title: Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity.
    Article Snippet: Article Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity

    Plasmid Preparation:

    Article Title: Hypoxia restores the acidosis-induced inhibition of cancer cell dissemination.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Myr-HA-Akt1 (Ramaswamy et al.)49 Addgene, plasmid # 9008 mCherry-CRY2-iSH2 (Idevall Hagren O et al.)39 Addgene, plasmid # 66839 Software and algorithms ImageJ NIH https://imagej.net/software/fiji/ NIS-Elements Nikon https://www.microscope.healthcare.nikon.com/ products/software/nis-elements/softwareresources MATLAB (R2025b) MathWorks https://www.mathworks.com/products/ matlab.html bioRender bioRender https://www.biorender.com/ Seahorse Wave Desktop Agilent https://www.agilent.com/en/product/cell- analysis/real-time-cell-metabolic-analysis/ xf-software/seahorse-wave-desktop-software740897 Prism 10 GraphPad https://www.graphpad.com HCI software Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ cameras/software/imaging-software/U11158.html QuPath Bankhead et al.50 https://qupath.github.io/ Cell Reports 45, 116970, February 24, 2026 19 Unless otherwise indicated, the cell culture medium with prescribed pH was prepared as previously described.9,15,25 In brief, cell culture medium was supplemented with 25 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES; 15630080, Gibco) and 25 mM PIPES; P1851, Millipore Sigma), and pH was adjusted to 7.4 or 6.4 with addition of NaOH and HCl. .. In select experiments (Figures 2F, 2G, S4G; Figures S5A–S5C), an isotonic medium made from DMEM without NaHCO3 (12800-058, Gibco) and supplemented with either 16.5 mM NaHCO3, 20 mM NaCl (pHe = 7.4) or 2 mM NaHCO3, 2 mM HCl and 30 mM NaCl (pHe = 6.4) was used.

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Software:

    Article Title: Hypoxia restores the acidosis-induced inhibition of cancer cell dissemination.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Myr-HA-Akt1 (Ramaswamy et al.)49 Addgene, plasmid # 9008 mCherry-CRY2-iSH2 (Idevall Hagren O et al.)39 Addgene, plasmid # 66839 Software and algorithms ImageJ NIH https://imagej.net/software/fiji/ NIS-Elements Nikon https://www.microscope.healthcare.nikon.com/ products/software/nis-elements/softwareresources MATLAB (R2025b) MathWorks https://www.mathworks.com/products/ matlab.html bioRender bioRender https://www.biorender.com/ Seahorse Wave Desktop Agilent https://www.agilent.com/en/product/cell- analysis/real-time-cell-metabolic-analysis/ xf-software/seahorse-wave-desktop-software740897 Prism 10 GraphPad https://www.graphpad.com HCI software Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ cameras/software/imaging-software/U11158.html QuPath Bankhead et al.50 https://qupath.github.io/ Cell Reports 45, 116970, February 24, 2026 19 Unless otherwise indicated, the cell culture medium with prescribed pH was prepared as previously described.9,15,25 In brief, cell culture medium was supplemented with 25 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES; 15630080, Gibco) and 25 mM PIPES; P1851, Millipore Sigma), and pH was adjusted to 7.4 or 6.4 with addition of NaOH and HCl. .. In select experiments (Figures 2F, 2G, S4G; Figures S5A–S5C), an isotonic medium made from DMEM without NaHCO3 (12800-058, Gibco) and supplemented with either 16.5 mM NaHCO3, 20 mM NaCl (pHe = 7.4) or 2 mM NaHCO3, 2 mM HCl and 30 mM NaCl (pHe = 6.4) was used.

    Article Title: Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli S13 Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) DSM 10719 E. coli S13 Δtls (Kanamycin sensitive) This work E. coli S13 ΔrflP (Kanamycin sensitive) Laganenka et al, 2020 E. coli S13 ΔflhC This work E. coli UTI89 Mulvey et al, 2001 E. coli UTI89 Δtls (Kanamycin sensitive) This work E. coli MG1655 Blattner et al, 1997 E. coli MG1655 ΔfliA This work E. coli MG1655 ΔflhC Rudenko et al, 2019 E. coli RP437 Parkinson and Houts, 1982 E. coli RP437 Δtar tsr trg tap aer Ames et al, 2002 E. coli RP437 ΔcheR Lovdok et al, 2007 Mouse line: C57BL/ 6 Jackson Laboratory JAX:00066 Mouse line: C3H/ HeN Inotiv (formerly Envigo) 040 Recombinant DNA pKD46 Datsenko and Wanner, 2000 pCP20 Cherepanov and Wackernagel, 1995 pUA66 Zaslaver et al, 2006 pTrc99A Amann et al, 1988 pBAD33RBS Yuan et al, 2017 pVS1962 Ni et al, 2017 pVS1963 Zaslaver et al, 2006 pVS1970 Ni et al, 2017 pTrc99A-Tls This work Reagent/resource Reference or source Identifier or catalog number pTrc99A-TlsNTrgΔN (TrgΔN, aa 400-546 of Trg) This work pTrc99A-TlsNTarΔN (TarΔN, aa 390-553 of Tar) This work pTrc99A-TlsNTsrΔN (TsrΔN, aa 392-551 of Tsr) This work pTrc99A-TlsNTapΔN (TapΔN, aa 388-533 of Tap) This work pTrc99A-TlsNAerΔN (AerΔN, aa 382-506 of Aer) This work pTrc99A-TlsN This work pTrc99A-Tls-sfGFP This work pTrc99A-TlsNsfGFP This work pTrc99A-TlsNsfGFP(N149Y) This work pTrc99A-TlsNsfGFP(R206A) This work pVS2890 Scarinci and Sourjik, 2023 pTrc99A-TlsNmCherry This work pTrc99A-M(Gly)8mCherry This work pTrc99A-TlsN (R4D)-mCherry This work pTrc99A-TlsN (R4K)-mCherry This work pTrc99A-TlsN*mCherry This work pTrc99A-TlsmCherry This work pTrc99A-mCherryTls This work pTrc99A-Trg This work pBAD33RBS-FlhDCsfGFP This work pBAD33RBS-FlhDCsfGFP (FlhC K42E, K45E) This work pM965 Stecher et al, 2004 Antibodies Anti-GFP antibody produced in a rabbit Sigma-Aldrich SAB4701015 Goat Anti-Rabbit IgG Antibody, HRPconjugate Sigma-Aldrich 12-348 Oligonucleotides and other sequence-based reagents Cloning primer sequences See Appendix Table S1 Chemicals, enzymes, and other reagents Bacto Tryptone Gibco 211705 Sodium chloride Carl Roth 3957.1 Yeast extract Gibco 212720 Bacto Agar BD 214010 14 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 182.10.130.106. .. Reagent/resource Reference or source Identifier or catalog number Agarose HEEO ultraquality Carl Roth 2269.1 Kanamycin Sigma-Aldrich 25389-94-0 Ampicillin Carl Roth 29T3.1 Chlroramphenicol Sigma-Aldrich 56-75-7 IPTG Carl Roth 2316.1 Polydimethylsiloxane (PDMS) Dow Corning Sylgard184 101697 SacI-HF restriction enzyme New England Biolabs R3156S HindIII-HF restriction enzyme New England Biolabs R3104S Gibson Assembly Master Mix New England Biolabs E2611L Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S Isoflurane McKesson 803250 MacConkey Agar Fisher Scientific B12306 Software Geneious Prime Geneious Prime Software https://www.geneious.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements AR software Nikon https:// www.microscope.healthcare.nikon.com/ products/software/nis-elements/niselements-advanced-research R software R Foundation Statutes https://www.r-project.org/ GraphPad Prism 10 Graphpad Software https://www.graphpad.com/ Other Infinite M1000 PRO plate reader TECAN TI Eclipse phasecontrast microscope Nikon Ti-E inverted fluorescence microscope Nikon Chemiluminescence imager Intas LSM 880 confocal laser scanning microscope Carl Zeiss Stainless steel beads Qiagen 69989 Tissue lyzer Omni TH115 ..

    Article Title: Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity.
    Article Snippet: Article Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity

    Article Title: Protocol for 3D sub-10-nm multicolor expansion STED nanoscopy using small-molecule organic probes.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms ImageJ/Fiji Schneider et al. 3 https://imagej.nih.gov/ij/ NIS Elements Nikon https://industry.nikon.com MATLAB MathWorks https://ww2.mathworks.cn/ FluoRender University of Utah https://www.sci.utah.edu/cibc-software/fluorender.html Anaconda version 3 Anaconda https://www.anaconda.com/ Excel Microsoft https://www.microsoft.com/zh-cn/microsoft365/excel Probe stock solution Reagent Final concentration Amount Probe powder N/A N/A PBS(PH 7.4) N/A 300 μL Total 300 μL 300 μL The probe solution is stable for at least one month at 4 ◦ C in the dark. ..

    Cell Culture:

    Article Title: Hypoxia restores the acidosis-induced inhibition of cancer cell dissemination.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Myr-HA-Akt1 (Ramaswamy et al.)49 Addgene, plasmid # 9008 mCherry-CRY2-iSH2 (Idevall Hagren O et al.)39 Addgene, plasmid # 66839 Software and algorithms ImageJ NIH https://imagej.net/software/fiji/ NIS-Elements Nikon https://www.microscope.healthcare.nikon.com/ products/software/nis-elements/softwareresources MATLAB (R2025b) MathWorks https://www.mathworks.com/products/ matlab.html bioRender bioRender https://www.biorender.com/ Seahorse Wave Desktop Agilent https://www.agilent.com/en/product/cell- analysis/real-time-cell-metabolic-analysis/ xf-software/seahorse-wave-desktop-software740897 Prism 10 GraphPad https://www.graphpad.com HCI software Hamamatsu Photonics https://www.hamamatsu.com/jp/en/product/ cameras/software/imaging-software/U11158.html QuPath Bankhead et al.50 https://qupath.github.io/ Cell Reports 45, 116970, February 24, 2026 19 Unless otherwise indicated, the cell culture medium with prescribed pH was prepared as previously described.9,15,25 In brief, cell culture medium was supplemented with 25 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES; 15630080, Gibco) and 25 mM PIPES; P1851, Millipore Sigma), and pH was adjusted to 7.4 or 6.4 with addition of NaOH and HCl. .. In select experiments (Figures 2F, 2G, S4G; Figures S5A–S5C), an isotonic medium made from DMEM without NaHCO3 (12800-058, Gibco) and supplemented with either 16.5 mM NaHCO3, 20 mM NaCl (pHe = 7.4) or 2 mM NaHCO3, 2 mM HCl and 30 mM NaCl (pHe = 6.4) was used.

    Protease Inhibitor:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Enzyme-linked Immunosorbent Assay:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Clinical Proteomics:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Multiplex sample analysis:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    other:

    Article Title: Multidimensional Cellular Micro-Compartments to Model Invasive Lobular Carcinoma Dormancy
    Article Snippet: Images were quantified utilizing various manual and automatic approaches with Fiji (ImageJ), NIS-Elements imaging software (Nikon Instruments), and MATLAB.

    Article Title: Cadherin-1-mediated communication at the leader-follower boundary controls mouse breast tumor organoid collective migration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pCMV deltaR8.2 Addgene Cat# 79047 pCMV-VSV-G Addgene Cat# 8454 Software and algorithms Partek Flow software Partek https://www.partek.com/ R The R Project https://www.r-project.org/ FIJI Distribution of ImageJ https://imagej.net/software/fiji/downloads Seurat (v5.0.0) Satijalab https://satijalab.org/seurat/ NIS-Elements Imaging Nikon https://www.microscope.healthcare.nikon. com/products/software/nis-elements GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism/ MATLAB Mathworks https://www.mathworks.com/products/ matlab.html PIVLab PIVLab https://www.pivlab.de/ AutoCAD Autodesk https://www.autodesk.com/products/ autocad/overview COMSOL COMSOL https://www.comsol.com/ CompuCell3D software CompuCell3D https://compucell3d.org/ CurveAlign/CT-Fire Laboratory for Optical and Computational Instrumentation CurveAlign (https://loci.wisc.edu/ curvealign/) CT-Fire (https://loci.wisc.edu/ctfire/) Other EasySep Streptavidin RapidSpheres STEMCell Cat# 50001 Sylgard 184 elastomer + curing agent Fisher Scientific Cat# 1508840 Fluosphere Polystyrene Beads (1μM, 580/ 605) Invitrogen Cat# F13083 18 Cell Reports 44, 116631, December 23, 2025

    Article Title: Tissue-resident macrophages co-develop with myocardial tissue in human induced pluripotent stem cell-derived organoids
    Article Snippet: Three-dimensional reconstruction of the acquired image stacks was performed using Fiji (ImageJ) or Nikon NIS-Elements Confocal software.

    Mutagenesis:

    Article Title: Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli S13 Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) DSM 10719 E. coli S13 Δtls (Kanamycin sensitive) This work E. coli S13 ΔrflP (Kanamycin sensitive) Laganenka et al, 2020 E. coli S13 ΔflhC This work E. coli UTI89 Mulvey et al, 2001 E. coli UTI89 Δtls (Kanamycin sensitive) This work E. coli MG1655 Blattner et al, 1997 E. coli MG1655 ΔfliA This work E. coli MG1655 ΔflhC Rudenko et al, 2019 E. coli RP437 Parkinson and Houts, 1982 E. coli RP437 Δtar tsr trg tap aer Ames et al, 2002 E. coli RP437 ΔcheR Lovdok et al, 2007 Mouse line: C57BL/ 6 Jackson Laboratory JAX:00066 Mouse line: C3H/ HeN Inotiv (formerly Envigo) 040 Recombinant DNA pKD46 Datsenko and Wanner, 2000 pCP20 Cherepanov and Wackernagel, 1995 pUA66 Zaslaver et al, 2006 pTrc99A Amann et al, 1988 pBAD33RBS Yuan et al, 2017 pVS1962 Ni et al, 2017 pVS1963 Zaslaver et al, 2006 pVS1970 Ni et al, 2017 pTrc99A-Tls This work Reagent/resource Reference or source Identifier or catalog number pTrc99A-TlsNTrgΔN (TrgΔN, aa 400-546 of Trg) This work pTrc99A-TlsNTarΔN (TarΔN, aa 390-553 of Tar) This work pTrc99A-TlsNTsrΔN (TsrΔN, aa 392-551 of Tsr) This work pTrc99A-TlsNTapΔN (TapΔN, aa 388-533 of Tap) This work pTrc99A-TlsNAerΔN (AerΔN, aa 382-506 of Aer) This work pTrc99A-TlsN This work pTrc99A-Tls-sfGFP This work pTrc99A-TlsNsfGFP This work pTrc99A-TlsNsfGFP(N149Y) This work pTrc99A-TlsNsfGFP(R206A) This work pVS2890 Scarinci and Sourjik, 2023 pTrc99A-TlsNmCherry This work pTrc99A-M(Gly)8mCherry This work pTrc99A-TlsN (R4D)-mCherry This work pTrc99A-TlsN (R4K)-mCherry This work pTrc99A-TlsN*mCherry This work pTrc99A-TlsmCherry This work pTrc99A-mCherryTls This work pTrc99A-Trg This work pBAD33RBS-FlhDCsfGFP This work pBAD33RBS-FlhDCsfGFP (FlhC K42E, K45E) This work pM965 Stecher et al, 2004 Antibodies Anti-GFP antibody produced in a rabbit Sigma-Aldrich SAB4701015 Goat Anti-Rabbit IgG Antibody, HRPconjugate Sigma-Aldrich 12-348 Oligonucleotides and other sequence-based reagents Cloning primer sequences See Appendix Table S1 Chemicals, enzymes, and other reagents Bacto Tryptone Gibco 211705 Sodium chloride Carl Roth 3957.1 Yeast extract Gibco 212720 Bacto Agar BD 214010 14 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 182.10.130.106. .. Reagent/resource Reference or source Identifier or catalog number Agarose HEEO ultraquality Carl Roth 2269.1 Kanamycin Sigma-Aldrich 25389-94-0 Ampicillin Carl Roth 29T3.1 Chlroramphenicol Sigma-Aldrich 56-75-7 IPTG Carl Roth 2316.1 Polydimethylsiloxane (PDMS) Dow Corning Sylgard184 101697 SacI-HF restriction enzyme New England Biolabs R3156S HindIII-HF restriction enzyme New England Biolabs R3104S Gibson Assembly Master Mix New England Biolabs E2611L Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S Isoflurane McKesson 803250 MacConkey Agar Fisher Scientific B12306 Software Geneious Prime Geneious Prime Software https://www.geneious.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements AR software Nikon https:// www.microscope.healthcare.nikon.com/ products/software/nis-elements/niselements-advanced-research R software R Foundation Statutes https://www.r-project.org/ GraphPad Prism 10 Graphpad Software https://www.graphpad.com/ Other Infinite M1000 PRO plate reader TECAN TI Eclipse phasecontrast microscope Nikon Ti-E inverted fluorescence microscope Nikon Chemiluminescence imager Intas LSM 880 confocal laser scanning microscope Carl Zeiss Stainless steel beads Qiagen 69989 Tissue lyzer Omni TH115 ..

    Microscopy:

    Article Title: Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli S13 Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) DSM 10719 E. coli S13 Δtls (Kanamycin sensitive) This work E. coli S13 ΔrflP (Kanamycin sensitive) Laganenka et al, 2020 E. coli S13 ΔflhC This work E. coli UTI89 Mulvey et al, 2001 E. coli UTI89 Δtls (Kanamycin sensitive) This work E. coli MG1655 Blattner et al, 1997 E. coli MG1655 ΔfliA This work E. coli MG1655 ΔflhC Rudenko et al, 2019 E. coli RP437 Parkinson and Houts, 1982 E. coli RP437 Δtar tsr trg tap aer Ames et al, 2002 E. coli RP437 ΔcheR Lovdok et al, 2007 Mouse line: C57BL/ 6 Jackson Laboratory JAX:00066 Mouse line: C3H/ HeN Inotiv (formerly Envigo) 040 Recombinant DNA pKD46 Datsenko and Wanner, 2000 pCP20 Cherepanov and Wackernagel, 1995 pUA66 Zaslaver et al, 2006 pTrc99A Amann et al, 1988 pBAD33RBS Yuan et al, 2017 pVS1962 Ni et al, 2017 pVS1963 Zaslaver et al, 2006 pVS1970 Ni et al, 2017 pTrc99A-Tls This work Reagent/resource Reference or source Identifier or catalog number pTrc99A-TlsNTrgΔN (TrgΔN, aa 400-546 of Trg) This work pTrc99A-TlsNTarΔN (TarΔN, aa 390-553 of Tar) This work pTrc99A-TlsNTsrΔN (TsrΔN, aa 392-551 of Tsr) This work pTrc99A-TlsNTapΔN (TapΔN, aa 388-533 of Tap) This work pTrc99A-TlsNAerΔN (AerΔN, aa 382-506 of Aer) This work pTrc99A-TlsN This work pTrc99A-Tls-sfGFP This work pTrc99A-TlsNsfGFP This work pTrc99A-TlsNsfGFP(N149Y) This work pTrc99A-TlsNsfGFP(R206A) This work pVS2890 Scarinci and Sourjik, 2023 pTrc99A-TlsNmCherry This work pTrc99A-M(Gly)8mCherry This work pTrc99A-TlsN (R4D)-mCherry This work pTrc99A-TlsN (R4K)-mCherry This work pTrc99A-TlsN*mCherry This work pTrc99A-TlsmCherry This work pTrc99A-mCherryTls This work pTrc99A-Trg This work pBAD33RBS-FlhDCsfGFP This work pBAD33RBS-FlhDCsfGFP (FlhC K42E, K45E) This work pM965 Stecher et al, 2004 Antibodies Anti-GFP antibody produced in a rabbit Sigma-Aldrich SAB4701015 Goat Anti-Rabbit IgG Antibody, HRPconjugate Sigma-Aldrich 12-348 Oligonucleotides and other sequence-based reagents Cloning primer sequences See Appendix Table S1 Chemicals, enzymes, and other reagents Bacto Tryptone Gibco 211705 Sodium chloride Carl Roth 3957.1 Yeast extract Gibco 212720 Bacto Agar BD 214010 14 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 182.10.130.106. .. Reagent/resource Reference or source Identifier or catalog number Agarose HEEO ultraquality Carl Roth 2269.1 Kanamycin Sigma-Aldrich 25389-94-0 Ampicillin Carl Roth 29T3.1 Chlroramphenicol Sigma-Aldrich 56-75-7 IPTG Carl Roth 2316.1 Polydimethylsiloxane (PDMS) Dow Corning Sylgard184 101697 SacI-HF restriction enzyme New England Biolabs R3156S HindIII-HF restriction enzyme New England Biolabs R3104S Gibson Assembly Master Mix New England Biolabs E2611L Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S Isoflurane McKesson 803250 MacConkey Agar Fisher Scientific B12306 Software Geneious Prime Geneious Prime Software https://www.geneious.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements AR software Nikon https:// www.microscope.healthcare.nikon.com/ products/software/nis-elements/niselements-advanced-research R software R Foundation Statutes https://www.r-project.org/ GraphPad Prism 10 Graphpad Software https://www.graphpad.com/ Other Infinite M1000 PRO plate reader TECAN TI Eclipse phasecontrast microscope Nikon Ti-E inverted fluorescence microscope Nikon Chemiluminescence imager Intas LSM 880 confocal laser scanning microscope Carl Zeiss Stainless steel beads Qiagen 69989 Tissue lyzer Omni TH115 ..

    Fluorescence:

    Article Title: Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli S13 Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) DSM 10719 E. coli S13 Δtls (Kanamycin sensitive) This work E. coli S13 ΔrflP (Kanamycin sensitive) Laganenka et al, 2020 E. coli S13 ΔflhC This work E. coli UTI89 Mulvey et al, 2001 E. coli UTI89 Δtls (Kanamycin sensitive) This work E. coli MG1655 Blattner et al, 1997 E. coli MG1655 ΔfliA This work E. coli MG1655 ΔflhC Rudenko et al, 2019 E. coli RP437 Parkinson and Houts, 1982 E. coli RP437 Δtar tsr trg tap aer Ames et al, 2002 E. coli RP437 ΔcheR Lovdok et al, 2007 Mouse line: C57BL/ 6 Jackson Laboratory JAX:00066 Mouse line: C3H/ HeN Inotiv (formerly Envigo) 040 Recombinant DNA pKD46 Datsenko and Wanner, 2000 pCP20 Cherepanov and Wackernagel, 1995 pUA66 Zaslaver et al, 2006 pTrc99A Amann et al, 1988 pBAD33RBS Yuan et al, 2017 pVS1962 Ni et al, 2017 pVS1963 Zaslaver et al, 2006 pVS1970 Ni et al, 2017 pTrc99A-Tls This work Reagent/resource Reference or source Identifier or catalog number pTrc99A-TlsNTrgΔN (TrgΔN, aa 400-546 of Trg) This work pTrc99A-TlsNTarΔN (TarΔN, aa 390-553 of Tar) This work pTrc99A-TlsNTsrΔN (TsrΔN, aa 392-551 of Tsr) This work pTrc99A-TlsNTapΔN (TapΔN, aa 388-533 of Tap) This work pTrc99A-TlsNAerΔN (AerΔN, aa 382-506 of Aer) This work pTrc99A-TlsN This work pTrc99A-Tls-sfGFP This work pTrc99A-TlsNsfGFP This work pTrc99A-TlsNsfGFP(N149Y) This work pTrc99A-TlsNsfGFP(R206A) This work pVS2890 Scarinci and Sourjik, 2023 pTrc99A-TlsNmCherry This work pTrc99A-M(Gly)8mCherry This work pTrc99A-TlsN (R4D)-mCherry This work pTrc99A-TlsN (R4K)-mCherry This work pTrc99A-TlsN*mCherry This work pTrc99A-TlsmCherry This work pTrc99A-mCherryTls This work pTrc99A-Trg This work pBAD33RBS-FlhDCsfGFP This work pBAD33RBS-FlhDCsfGFP (FlhC K42E, K45E) This work pM965 Stecher et al, 2004 Antibodies Anti-GFP antibody produced in a rabbit Sigma-Aldrich SAB4701015 Goat Anti-Rabbit IgG Antibody, HRPconjugate Sigma-Aldrich 12-348 Oligonucleotides and other sequence-based reagents Cloning primer sequences See Appendix Table S1 Chemicals, enzymes, and other reagents Bacto Tryptone Gibco 211705 Sodium chloride Carl Roth 3957.1 Yeast extract Gibco 212720 Bacto Agar BD 214010 14 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 182.10.130.106. .. Reagent/resource Reference or source Identifier or catalog number Agarose HEEO ultraquality Carl Roth 2269.1 Kanamycin Sigma-Aldrich 25389-94-0 Ampicillin Carl Roth 29T3.1 Chlroramphenicol Sigma-Aldrich 56-75-7 IPTG Carl Roth 2316.1 Polydimethylsiloxane (PDMS) Dow Corning Sylgard184 101697 SacI-HF restriction enzyme New England Biolabs R3156S HindIII-HF restriction enzyme New England Biolabs R3104S Gibson Assembly Master Mix New England Biolabs E2611L Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S Isoflurane McKesson 803250 MacConkey Agar Fisher Scientific B12306 Software Geneious Prime Geneious Prime Software https://www.geneious.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements AR software Nikon https:// www.microscope.healthcare.nikon.com/ products/software/nis-elements/niselements-advanced-research R software R Foundation Statutes https://www.r-project.org/ GraphPad Prism 10 Graphpad Software https://www.graphpad.com/ Other Infinite M1000 PRO plate reader TECAN TI Eclipse phasecontrast microscope Nikon Ti-E inverted fluorescence microscope Nikon Chemiluminescence imager Intas LSM 880 confocal laser scanning microscope Carl Zeiss Stainless steel beads Qiagen 69989 Tissue lyzer Omni TH115 ..

    Laser-Scanning Microscopy:

    Article Title: Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli S13 Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) DSM 10719 E. coli S13 Δtls (Kanamycin sensitive) This work E. coli S13 ΔrflP (Kanamycin sensitive) Laganenka et al, 2020 E. coli S13 ΔflhC This work E. coli UTI89 Mulvey et al, 2001 E. coli UTI89 Δtls (Kanamycin sensitive) This work E. coli MG1655 Blattner et al, 1997 E. coli MG1655 ΔfliA This work E. coli MG1655 ΔflhC Rudenko et al, 2019 E. coli RP437 Parkinson and Houts, 1982 E. coli RP437 Δtar tsr trg tap aer Ames et al, 2002 E. coli RP437 ΔcheR Lovdok et al, 2007 Mouse line: C57BL/ 6 Jackson Laboratory JAX:00066 Mouse line: C3H/ HeN Inotiv (formerly Envigo) 040 Recombinant DNA pKD46 Datsenko and Wanner, 2000 pCP20 Cherepanov and Wackernagel, 1995 pUA66 Zaslaver et al, 2006 pTrc99A Amann et al, 1988 pBAD33RBS Yuan et al, 2017 pVS1962 Ni et al, 2017 pVS1963 Zaslaver et al, 2006 pVS1970 Ni et al, 2017 pTrc99A-Tls This work Reagent/resource Reference or source Identifier or catalog number pTrc99A-TlsNTrgΔN (TrgΔN, aa 400-546 of Trg) This work pTrc99A-TlsNTarΔN (TarΔN, aa 390-553 of Tar) This work pTrc99A-TlsNTsrΔN (TsrΔN, aa 392-551 of Tsr) This work pTrc99A-TlsNTapΔN (TapΔN, aa 388-533 of Tap) This work pTrc99A-TlsNAerΔN (AerΔN, aa 382-506 of Aer) This work pTrc99A-TlsN This work pTrc99A-Tls-sfGFP This work pTrc99A-TlsNsfGFP This work pTrc99A-TlsNsfGFP(N149Y) This work pTrc99A-TlsNsfGFP(R206A) This work pVS2890 Scarinci and Sourjik, 2023 pTrc99A-TlsNmCherry This work pTrc99A-M(Gly)8mCherry This work pTrc99A-TlsN (R4D)-mCherry This work pTrc99A-TlsN (R4K)-mCherry This work pTrc99A-TlsN*mCherry This work pTrc99A-TlsmCherry This work pTrc99A-mCherryTls This work pTrc99A-Trg This work pBAD33RBS-FlhDCsfGFP This work pBAD33RBS-FlhDCsfGFP (FlhC K42E, K45E) This work pM965 Stecher et al, 2004 Antibodies Anti-GFP antibody produced in a rabbit Sigma-Aldrich SAB4701015 Goat Anti-Rabbit IgG Antibody, HRPconjugate Sigma-Aldrich 12-348 Oligonucleotides and other sequence-based reagents Cloning primer sequences See Appendix Table S1 Chemicals, enzymes, and other reagents Bacto Tryptone Gibco 211705 Sodium chloride Carl Roth 3957.1 Yeast extract Gibco 212720 Bacto Agar BD 214010 14 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 182.10.130.106. .. Reagent/resource Reference or source Identifier or catalog number Agarose HEEO ultraquality Carl Roth 2269.1 Kanamycin Sigma-Aldrich 25389-94-0 Ampicillin Carl Roth 29T3.1 Chlroramphenicol Sigma-Aldrich 56-75-7 IPTG Carl Roth 2316.1 Polydimethylsiloxane (PDMS) Dow Corning Sylgard184 101697 SacI-HF restriction enzyme New England Biolabs R3156S HindIII-HF restriction enzyme New England Biolabs R3104S Gibson Assembly Master Mix New England Biolabs E2611L Q5 Site-Directed Mutagenesis Kit New England Biolabs E0554S Isoflurane McKesson 803250 MacConkey Agar Fisher Scientific B12306 Software Geneious Prime Geneious Prime Software https://www.geneious.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/ NIS Elements AR software Nikon https:// www.microscope.healthcare.nikon.com/ products/software/nis-elements/niselements-advanced-research R software R Foundation Statutes https://www.r-project.org/ GraphPad Prism 10 Graphpad Software https://www.graphpad.com/ Other Infinite M1000 PRO plate reader TECAN TI Eclipse phasecontrast microscope Nikon Ti-E inverted fluorescence microscope Nikon Chemiluminescence imager Intas LSM 880 confocal laser scanning microscope Carl Zeiss Stainless steel beads Qiagen 69989 Tissue lyzer Omni TH115 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity.
    Article Snippet: Article Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity

    Imaging:

    Article Title: Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity.
    Article Snippet: Article Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity

    Concentration Assay:

    Article Title: Protocol for 3D sub-10-nm multicolor expansion STED nanoscopy using small-molecule organic probes.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms ImageJ/Fiji Schneider et al. 3 https://imagej.nih.gov/ij/ NIS Elements Nikon https://industry.nikon.com MATLAB MathWorks https://ww2.mathworks.cn/ FluoRender University of Utah https://www.sci.utah.edu/cibc-software/fluorender.html Anaconda version 3 Anaconda https://www.anaconda.com/ Excel Microsoft https://www.microsoft.com/zh-cn/microsoft365/excel Probe stock solution Reagent Final concentration Amount Probe powder N/A N/A PBS(PH 7.4) N/A 300 μL Total 300 μL 300 μL The probe solution is stable for at least one month at 4 ◦ C in the dark. ..



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